Cultivation and differentiation of human umbilical cord-derived mesenchymal stem cells treated with amphotericin B

doi:10.3969/j.issn.2095-4344.2014.28.009

Abstract

Abstract:

BACKGROUND: High-quality and efficient umbilical cord-derived mesenchymal stem cells could be obtained by establishing and improving the method to avoid fungal contamination and by effectively decreasing pollution rate of isolated culture during umbilical cord collection.
OBJECTIVE: To explore the effects of amphotericin B on growth and differentiation potential of umbilical cord-derived mesenchymal stem cells.
METHODS: Umbilical cord-derived mesenchymal stem cells were separated from healthy full-termed delivery fetus using collagenase digestion method and treated with amphotericin B. Subsequently, umbilical cord-derived mesenchymal stem cells were amplified and cultured in vitro with MesenPRO RSTM medium. The third passage of umbilical cord-derived mesenchymal stem cells in logarithmic phase was obtained to analyze their morphology, proliferation and immunophenotype, and induced to differentiate into osteoblasts and adipocytes in vitro.
RESULTS AND CONCLUSION: After treatment with amphotericin B, umbilical cord-derived mesenchymal stem cells were successfully isolated and cultured in vitro. Flow cytometry results revealed that human umbilical cord-derived mesenchymal stem cells strongly expressed CD44, CD105 and CD73, CD90, and negatively expressed HLA-DR, CD29, CD31, and CD34. Amphotericin B-treated human umbilical cord-derived mesenchymal stem cells can still differentiate into adipocytes and osteoblasts in vitro.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: umbilical cord, mesenchymal stem cells, amphotericin B, fungi

CLC Number:

R394.2

Huang Wen-jing, Liu Jun-jiang, Zhou Jian-yu, Hong Jing-xin, Li Qian, Han Jun-ling. Cultivation and differentiation of human umbilical cord-derived mesenchymal stem cells treated with amphotericin B[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4479-4484.

Figures/Tables 6

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